TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| SM10 λPir | Conjugation-competent strain | 44 |
| SY327 λPir | Routine cloning | 44 |
| DH5α | Routine cloning | 7 |
| BL21-DE | Protein expression in E. coli | 68 |
| Yersinia pseudotuberculosis | ||
| IP2666 pYV (pIBI) | Wild-type Y. pseudotuberculosis | 27 |
| ΔyopB | Deletion of yopB (codons 19-346) in IP2666 | M. Fisher, unpublished |
| ΔyscF | Deletion of yscF (codons 2-86) in IP2666 | This study |
| ΔyopN | Deletion of yopN (codons 2-287) in IP2666 | This study |
| ΔlcrV | Deletion of lcrV (codons 19-346) in YPIII | J. M. Balada, unpublished |
| Plasmids | ||
| pGEM-T easy | Cloning of PCR products, Apr | Promega |
| pCR2.1 | Cloning of PCR products, Apr, Kmr | Invitrogen |
| pCVD442 | Suicide vector, Apr | 14 |
| pTRC99A | IPTG-inducible expression vector, Apr | 1 |
| pGEX-5X-1 | GST fusion expression vector, Apr | Amersham |
| pGEM-yscF | Template for error-prone PCR | This study |
| pCVD442-ΔyscF | Suicide vector for deleting yscF | This study |
| pCVD442-ΔyopN | Suicide vector for deleting yopN | This study |
| pGEX-yscF | GST-YscF fusion expression vector | This study |
| pGEX-YopD | GST-YopD fusion expression vector | This study |
| pTRC99A-yscF | Expresses yscF from tac promoter | This study |