TABLE 3.
RhaS or RhaR derivative | β-Galactosidase activitya
|
|
---|---|---|
−l-Rhamnose | +l-Rhamnose | |
RhaS derivatives | ||
Wild type | 0.32 | 179 |
N168P/S169P | 0.47 | 55 |
E167G/S169G/S171G | 0.24 | 206 |
E167A/S169A/S171A | 0.08 | 120 |
RhaS with RhaR linker | 0.31 | 25 |
RhaR derivatives | ||
Wild type | 3.3 | 16 |
RhaR with RhaS linker | 1 | 8.4 |