TABLE 2.
Arylsulfatase activities of flgP- and flgQ-astA transcriptional fusions in σ28 and σ54 mutants
| Strain | Arylsulfatase unitsa (mean ± SD)
|
|
|---|---|---|
| flgP::astAb | flgQ::astAc | |
| Wild type | 282.7 ± 40.0 | 29.3 ± 1.1 |
| ΔfliA | 308.1 ± 22.9 | 29.0 ± 0.7 |
| ΔrpoN | 163.9 ± 1.5 | 10.6 ± 0.9 |
| ΔrpoN/vector | 155.6 ± 6.3 | 11.4 ± 0.8 |
| ΔrpoN/rpoN | 215.4 ± 4.2 | 19.1 ± 1.2 |
a
Results are from a typical assay with determinations for each sample performed in triplicate. One unit equals the amount of arylsulfatase required to generate 1 nmol of nitrophenol per hour per OD600 unit.
b
Strains used were SMS341, SMS350, SMS420, SMS524, and SMS526.
c
Strains used were SMS281, SMS323, SMS335, SMS532, and SMS534.