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. 2006 Oct 20;189(1):187–197. doi: 10.1128/JB.01273-06

FIG. 6.

FIG. 6.

Genetic organization and characterization of the promoter of the yrrT operon. (A) The genetic organization of the yrrT operon is presented. The −35 and −10 regions are in uppercase letters, and the CymR binding site is indicated by a black box. (B) Northern blot analysis of the yrrT operon. B. subtilis 168 was grown in minimal medium with 1 mM sulfate (lane 1) or with 1 mM methionine (lane 2) as sulfur sources. The hybridization was performed with an yrhB-specific probe, which is indicated panel A. The arrow indicates the apparent size of the transcript detected. (C) Identification of the transcription start site of the yrrT operon by primer extension. Total RNA was extracted from B. subtilis 168 grown in minimal medium with 1 mM sulfate (lane 1) or 1 mM methionine (lane 2) as the sole sulfur source. The size of the extended product was compared to a DNA sequencing ladder of the yrrT promoter region. An asterisk marks the +1 site.