TABLE 1.
Strains used in this studya
| B. subtilis strain | Genotype | Source or reference |
|---|---|---|
| 168 | trpC2 | Laboratory stock |
| BSIP1144 | trpC2 amyE::pΔA(−108, +126) yrrT′-lacZ cat | 18 |
| BSIP1165 | trpC2 yrhB::aphA3 | pDIA5533 → 168 |
| BSIP1303 | trpC2 ΔyrhAB::aphA3 | This study |
| BSIP1304 | trpC2 ΔcysK::spc | 6 |
| BSIP1305 | trpC2 ΔyrhAB::aphA3 ΔcysK::spc | BSIP1304 → BSIP1303 |
| BSIP1314 | trpC2 amyE::(pΔAyrrT′-lacZ) ΔcysK::spc | BSIP1304 → BSIP1144 |
| BSIP1758 | trpC2 ΔluxS::cat | This study |
| BSIP1794 | trpC2 amyE::(pΔA yrrT′-lacZ cat) ΔcymR | 18 |
| BSIP1837 | trpC2 purA26 cysE14 amyE::(pΔA yrrT′-lacZ cat) | pDIA5512 → QB944 |
| BSIP1840 | trpC2 purA26 cysE14 ΔcysK::spc amyE::(pΔA yrrT′-lacZ cat) | DNA BSIP1304 → BSIP1837 |
| BSIP1870 | trpC2 yrhA′::lacZ-erm ΔyrhA ΔcysK::spc | DNA BSIP1304 → BFS2063 |
| BFS2063 | trpC2 yrhA′::lacZ-erm ΔyrhA | W. Schumann (27) |
| QB944 | trpC2 purA26 cysE14 | 17 |
a
Arrows indicate construction by transformation. cat is the pC194 chloramphenicol acetyltransferase gene, aphA3 is a kanamycin resistance gene, spc is a spectinomycin resistance gene, and erm is the pMutin erythromycin resistance gene. BFS2063 was constructed during the Bacillus Functional Analysis Program using the pMutin4 system (27).