FIG. 4.

Northern analysis of ntdABC transcription. (A) Structure of the ntdABC operon. Promoters and transcriptional terminators are indicated. The two transcripts of the ntdABC operon and the probes used for Northern analysis are shown. (B) Northern analysis of the ntdABC transcript using probes for ntdC and glcP. B. subtilis strains 61884 (wild-type) and 84R5 (rpoB5 mutant) were grown in S7N medium containing excess (1%) glucose until OD₆₅₀ reached 0.5, after which total cellular RNA was prepared from each strain using Isogen reagent. RNA samples (2 or 10 μg) were subjected to electrophoresis, transferred to a membrane, and then hybridized with the RNA probe for ntdC (left panel) or glcP (right panel) shown in panel A. Lane wt, wild type.