TABLE 3.
Virulence and physiologically important genes with altered sequences in strain D39 compared to strain R6a
| D39 locus tag | Gene | Function | Source or referenceb |
|---|---|---|---|
| SPD_0063 | strH | β-N-Acetylhexosaminidase | STM (43) |
| SPD_0065 | bgaC | β-Galactosidase | 52 |
| SPD_0080 | SSURE fibronectin binding | 21 | |
| SPD_0081 | rr08 | Response regulator | 63, 111 |
| SPD_0168 | ribE | Riboflavin synthesis | 71 |
| SPD_0315-SPD_0323 | Δcps | Capsule biosynthesis | 3, 69 |
| SPD_0336 | pbp1A | Penicillin-binding protein transglycosylase/transpeptidase | 49, 64, 90 |
| SPD_0479 | nusA | Transcription termination/antitermination | 18 |
| SPD_0636 | spxB | Pyruvate oxidase | STM/experimental (12, 65, 87, 91, 92, 106) |
| SPD_0660 | ftsX | ABC transporter/cell division | 102 |
| SPD_0665 | pyrDA | Dihydro-orotate dehydrogenase (pyrimidine biosynthesis) | 5 |
| SPD_0773 | fruA | Fructose PTS | STM (43) |
| SPD_0854 | flpA(pavA) | Fibronectin binding | STM/experimental (47, 64, 95) |
| SPD_0967 | murA1 | Homolog of MurA (first step in murein biosynthesis) | 19, 70 |
| SPD_1131 | carB | Carbamoylphosphate synthase, heavy subunit (pyrimidine biosynthesis) | 73 |
| SPD_1337 | atpA | Proton-translocating ATPase, F1 α subunit | 32 |
| SPD_1346 | Hypothetical | STM (43) | |
| SPD_1461 | psaB | Manganese transport | Experimental (24, 27, 72, 75, 85) |
| SPD_1462 | psaC | Manganese transport | Experimental (24, 27, 72, 75, 85) |
| SPD_1512 | secA | Preprotein translocase subunit | 93 |
| SPD_1671 | amiA | Oligopeptide transport | 2, 57 |
| SPD_1740 | cinA | Competence induced | STM (43) |
| SPD_1758 | rpoC | RNA polymerase β′ subunit | 116 |
| SPD_1797 | ccpA | Catabolite control | STM/experimental (37, 53) |
| SPD_1961 | Hypothetical (putative transcription regulator) | STM (43) | |
| SPD_1965 | pcpA | Choline-binding protein | STM/experimental (43, 101) |
| SPD_1974 | Conserved hypothetical | STM (43) | |
| SPD_1987 | Hypothetical (fucolectin-related protein) | STM (43) | |
| SPD_2005 | dltA | d-Ala ligase | STM (43) |
| SPD_2012 | glpO | α-Glycerophosphate oxidase | 98 |
| SPD_2022 | clpC | Stress-related ATPase | Experimental (22, 23, 51, 96, 97) |
| SPD_2028 | cbpD | Murein hydrolase | STM/experimental (, 40, 43, 46, 56) |
a
Specific mutations are listed in Tables S1 and S3 in the supplemental material.
b
Evidence for a role in virulence exists where indicated. STM, signature-tagged mutagenesis.