FIG. 5.
Inhibition of cell division by MinCC is dependent on MinD. (A to D) Micrographs of WM1032 (ΔminCDE) cells carrying plasmids expressing his-tagged minD (pWM2691) from an arabinose-inducible promoter and either FLAG-minC (pWM2801) or FLAG-minCC (pWM2844) from IPTG-inducible promoters. Cells were grown in 0.1 mM IPTG (for minCC, expressed from a stronger promoter) or 1 mM IPTG (for minC, expressed from a weaker promoter) and either without (A and C) or with (B and D) 0.2% arabinose (for expression of minD) at 30°C. Bar, 10 μm. (E) Immunoblots of extracts from the cells in panels A to D, probed with anti-FLAG to detect FLAG-MinC or FLAG-MinCC (top) or anti-MinD to detect MinD in the corresponding extracts (bottom). The relative intensities of the FLAG-MinC and FLAG-MinCC bands from extracts of the filamentous cells in 0.2% arabinose are shown below the anti-FLAG blot. Protein size markers are on the left.