TABLE 2.

Oligonucleotides used for plasmid construction

Name	Sequence
pDSW-6His	CGGAATTCCACCATCACCATCACCATGAGCTCGGTACCCGGGGATCC
pDSW-FLAG	CGGAATTCGACTACAAGGACGACGATGACAAAGAGCTCGGTACCCGGGGATCC
pDSW-reverse	CAAATTCTGTTTTATCAGAC
MinC-f (SacI)	GCGAGCTCTCAAACACGCCAATCGAGC
MinC122-f (SacI)	GCGAGCTCGTCACAAAAACGCGTTTAATAG
MinC-r (BamHI)	CGGGATCCTCAATTTAACGGTTGAACGG
MinE-r (BamHI)	CGCGGATCCTTTCAGCTCTTCTGCTTC
MinE-r2 (BamHI)	CGGGATCCTTATTTCAGCTCTTCTGCTTCC
MinC-R172A-f	CATGATGCGCGGTGCTGCGCTGGCAGGGG
MinC-R172A-r	CCCCTGCCAGCGCAGCACCGCGCATCATG
MinC-r (SalI)	ACGCGTCGACATTTAACGG
MinD6	TTGAATTCGCACGCATTATTGTTG