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Survival of P. putida and its ΔrpoT mutant upon sudden temperature shock. (A) Wild-type DOT-T1E and (B) DOT-T1E-ΔrpoT. Cells were grown in 40 ml LB medium at 30°C, and when indicated, four aliquots were made and incubated at 30°C (⧫), 37°C (•), 42°C (▴), and 50°C (▪). The viable cells were counted at the indicated times. The data are the averages of six determinations, with standard deviations below 5% of the given values.
