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. 2006 Sep 15;189(1):109–118. doi: 10.1128/JB.00710-06

FIG. 1.

FIG. 1.

Genetic map of the S. marcescens pirin gene locus and construction of the pirinSm gene deletion mutant S. marcescens PC103. (A) Genetic maps of S. marcescens Db11 and CH-1 pirin gene loci. The LysM domain (2) and AraC family (29) are proteins encoded, respectively, by the two genes identified upstream and downstream of the pirinSm gene. (B) Construction of the pirinSm gene knockout mutant strain PC103 by insertion of a Sm resistance gene Ω cassette into the S. marcescens CH-1 pirin gene through homologous recombination (28). pSC16 is a recombinant suicide vector for insertion-deletion mutagenesis of the pirinSm gene. (C) Western blot analysis of pirinSm production. Panel i, whole-cell crude extracts were separated by 12.5% SDS-PAGE and subjected to Coomassie brilliant blue staining. Panel ii, results of pirinSm detection using anti-pirinSm polyclonal antibodies. Lane 1, S. marcescens CH-1; lane 2, S. marcescens PC103; lane 3, S. marcescens PC103 transformed with pSC15(pBAD18-Kan::pirinSm); lane M, protein marker.