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. 2006 Nov 1;81(1):237–247. doi: 10.1128/JVI.01590-06

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Copyright © 2007, American Society for Microbiology

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FIG. 6. — A single passage of mosquito-cell-derived virus (mos-RRV) through mammalian cells (generating mam-RRV) restores type I IFN induction in mDCs. A. Myeloid DCs were infected with mam-RRV, mos-RRV, or mos-RRV passaged a single time back through BHK-21 cells. Infection percentages were calculated for GFP-positive cells by flow cytometry at 12 h postinfection. B. Supernatants from each of the corresponding samples were evaluated by type I IFN bioassay. Data are from a representative experiment and are presented as the mean and standard error of the mean of three independent samples. mam-RRV compared to mos-RRV and mos-RRV compared to mos-RRV passaged through mammalian cells (mos-RRV → mam-RRV) were statistically significant (P < 0.05) as determined by one-way ANOVA for the experiments in both panels A and B. mam-RRV compared to mos-RRV → mam-RRV was not statistically significant as determined by one-way ANOVA for both panels A and B.