FIG. 7.

Mosquito-cell-derived VEE virus and BFV induce less type I IFN than mammalian-cell-derived viruses on mDCs. A. Myeloid DCs were infected with mam- and mos-VEE at an MOI of 8.0, and infectivity was calculated by flow cytometry based on GFP expression at 12 h postinfection. B. Supernatants from the same samples as in panel A were analyzed by type I IFN bioassay. C. mos-BFV (strain 10E101SC) with E2 N-linked glycans induces less type I IFN than mam-BFV at 12 h postinfection by IFN bioassay. Data in panels A and B represent the mean and standard error of the mean of three independent samples and are representative of results from two independent experiments. Differences between mam- and mos-VEE were statistically significant (P < 0.05) as determined by one-way ANOVA (A and B).