FIG. 5.

Interactions between TAP/gE fusion proteins and HSV proteins. Human HaCaT keratinocytes were infected with Ad expressing either TAP/gE550 or TAP/gE470 for 18 h and then infected with F-gEΔCT (a gE-null mutant) for an additional 7 to 8 h. The cells were radiolabeled with [³⁵S]methionine-cysteine for 3 h, and then cell extracts were made using 0.5% NP-40 lysis buffer or 1% digitonin lysis buffer and low salt (L) (100 mM NaCl) or high salt (H) (500 mM NaCl). Extracts were centrifuged at 60,000 × g and supernatants incubated with IgG-Sepharose at 4°C. Proteins were eluted and subjected to electrophoresis. Two sets of bands, designated VP22* and UL11*, were observed when cells were infected with TAP/gE550 and F-gEΔCT but not with TAP/gE470 and F-gEΔCT and not without F-gEΔCT. Molecular mass markers of 97, 66, 46 and 30 kDa are indicated.