FIG. 7.

Coimmunoprecipitation of VP16, VP22, or UL11 with gE from HSV-infected cells. HaCaT cells were infected with HSV F-BAC (w.t.) expressing wild-type gE, F-BAC gE519, F-BAC gE495, F-BAC gE470, F-BAC gE448, or F-BAC gE⁻ for 12 h. Cell extracts were made using 0.5% NP-40 lysis buffer and gE immunoprecipitated with anti-gE MAb 3114. Precipitated proteins and a sample representing 20% of the cell lysate were subjected to electrophoresis, and proteins were transferred to membranes and then Western blotted with rabbit polyclonal antibodies specific for anti-VP16 (panel A, top), anti-VP22 (panel A, middle), anti-UL11 (panel A, bottom), or gE (B). VP22 and UL11 were quantified using IP Lab Gel software, with the background value (set at 1.0) determined by counting the pixels in the blot regions containing no obvious proteins.