FIG. 9.

VP22 binds to gE/gI and gD in the absence of other HSV proteins. HaCaT cells were infected for 24 h with nonreplicating Ad vectors expressing VP22, gE/gI, and gD in conjunction with AdTet-trans, as indicated in the upper panel. Cell extracts were made using 0.5% NP-40 lysis buffer. (A) gE/gI immunoprecipitated with pooled gE-specific MAb 3114 and gI-specific MAb 3104 (Anti-gE/gI) or gD precipitated with anti-gD MAb DL6 (Anti-gD), as indicated. Immunoprecipitated proteins were probed using antibodies specific to VP22. (B) Approximately 5% of the cell extract was subjected to electrophoresis, transferred to membranes, and then blotted with anti-VP22 antibodies.