FIG. 5.

RSV-F heparin-binding peptides inhibit A2 binding to Vero cells. Binding of purified A2 in the presence of RSV-F HBD peptides was examined on Vero monolayers. Peptide (at saturation binding) was mixed with purified A2 used at 50% of saturation and then incubated on Vero cells for 1 h prior to washing and fixing with 4% paraformaldehyde. Monolayers were air dried and then blocked with 5% BSA-PBS-Tween and incubated overnight with an RSV-F monoclonal antibody pool, and virus binding was detected by enzyme-linked immunosorbent assay using goat anti-mouse horseradish peroxidase. Data compare binding of virus in peptide-containing wells to binding in the absence of peptide and are representative of one of at least two experiments, with error bars indicating the standard deviations of the means of duplicate wells. *, P < 0.05.