FIG. 2.
In vivo expression of the antigen. (A) Mice were immunized with rSmeg-gp120 (5 × 107 CFU), and both the liver and spleen were removed from mice at 1, 7, and 14 days following immunization. Organ homogenates were plated in the presence or absence of kanamycin to determined CFU numbers. Data are the means of results from five mice per group ± SE. (B) Mice were immunized with rSmeg-luc (5 × 107 CFU) or DNA-luc (50 μg), and the levels of luciferase protein expression were measured over time. (C) Mice were either only primed or primed and boosted 6 weeks later, with rSmeg-luc and the levels of luciferase expression being measured over time. Data are the means of results from five mice per group ± SE. *, P < 0.001, compared to the luciferase expression following the priming immunization.