FIG. 2.
HIV-1 and MMLV infectivities in siRNA-treated HeLa-P4 cells. (A) Normalized HIV-Luc infectivities following transfection with the indicated siRNAs. Values in empty bars were derived from cells infected with 2 × 106 reverse transcriptase cpm, whereas the filled-in bars were from infections initiated with 4 × 105 reverse transcriptase cpm. (B) Normalized luciferase values in cell extracts following infection with an equal volume (350 μl) of MLV-Luc. Mock, cells infected following treatment with oligofectamine reagent in the absence of siRNA; HeLaP4, infection of unmanipulated cells. Prior to infection, cell cultures were processed to generate the data presented in Fig. 1. Results are representative of a minimum of two independent experiments.