FIG. 1.

Deficient innate MCMV immunity in MA/My.L-H2^b. (A) The chromosome 17 interval from D17Mit57 to D17Mit93 in M.L-H2^b is depicted at top with informative SSLP markers used in genetic screening and selection also shown. Physical map locations (in megabases [Mb]) are indicated. The H-2 complex and K and D class I genes are also shown. Virus levels in MCMV-infected (d 3.5) M.L-H2^b, L.M-H2^k, and control mice were quantitated by QPCR (B) or virus plaque assay (C). The plaque assay detection level is indicated by the broken line in panel C. Four to eight animals were studied per group. (D) Spleen (filled symbols) and liver (open symbols) virus levels also were quantitated for similarly infected (MA/My × C57L)F₁ and M.L-H-2^b/k mice and their noncongenic H-2^k littermates (LM control), and for C57L control mice, by QPCR. (E) Spleen virus levels in MCMV-infected (M.L-H2^bR1 × MA/My) × C57L hybrids segregated by H-2 genotype (as designated) and control strains quantitated by QPCR are shown. Data are representative of two independent experiments.