TABLE 1.
Vaccination and challenge regimens
| Vaccine regimen | n | Animal nos.a | Subtype AE SHIV immunization at:
|
|||
|---|---|---|---|---|---|---|
| Week 0 | Week 4 | Week 8 | Week 22 (all groups) | |||
| Control | 6 | 6167, 6352, 5350, 6264, 5620, 6376 | Control pHIS-empty (1 mg, i.m.) | Control pHIS-empty (1 mg, i.m.) | Control FPV-M3 (3 × 108 PFU, i.m.) | |
| Two-DNA/FPV-hi | 6 | 5396, 5616, 6279, 5085, 5912, 5396 | DNA pHIS-SHIV-AE (1 mg, i.m.) | DNA (1 mg) pHIS-SHIV-AE | FPV-SHIV-AE (3 × 108 PFU, i.m.) | |
| Two-DNA/FPV | 6 | 6276, 6370, 5023, 6284, 6263, 6353 | DNA pHIS-SHIV-AE (1 mg, i.m.) | DNA pHIS-SHIV-AE (1 mg, i.m.) | FPV-SHIV-AE (5 × 107 PFU, i.m.) | SHIVmn229 (subtype B) challenge (105 TCID50, intrarectal) |
| Three-DNA | 6 | 5614, 6351, 5618, 6364, 6173, 6371 | DNA pHIS-SHIV-AE (1 mg, i.m.) | DNA pHIS-SHIV-AE (1 mg, i.m.) | DNA pHIS-SHIV-AE (1 mg, i.m.) | |
| VV/FPV | 6 | 6349, 6377, 6259, 6363, 6262, 6388 | VV-SIV Gag/Pol, VV-HIV-1AE Env, VV-HIV-1AE Tat/Rev (each, 108 PFU, i.m.) | FPV-SHIV-AE (3 × 108 PFU, i.m.) | ||
a
Animal numbers in bold indicate those that expressed the Mane-A*10 allele and were studied with the Mane-A*10/KP9 tetramer.