FIG. 1.

Predicted Ad5 E1B region and its gene products. The region and various E1B landmarks are shown at the top. Numbers refer to nucleotides in the Ad5 sequence. Four differentially spliced mRNAs are depicted below as thick lines. Their lengths are noted on the right. Thin printed arcs represent introns between splice donor (SD) and acceptor (SA) sites. The five open reading frames encoding the E1B proteins are illustrated as boxes next to their corresponding mRNA. The shading of the boxes (gray, black, and white) represents the used reading frames 1 to 3, respectively. The gene products are named with reference to their numbers of amino acid residues. E1B-176R and E1B-496R therefore represent E1B-19K and E1B-55K. The open reading frames encoding E1B-156R, E1B-93R, and E1B-84R are generated through fusion of two exons. The eliminated intron sequences are illustrated as dashed lines. Within the box representing the E1B-55K protein, some protein hallmarks are noted with black bars marking their positions within the primary sequence: the nuclear export sequence (NES) at position 83 to 93; the SUMO1 conjugation motif (SCM) at 104 to 106; the repression domain (RD) around amino acid R443; and the three phosphorylation sites at S490, S491, and T495 (P3). This figure was produced with information from reference 60. The nucleotide numbering is according to the published Ad5 sequence from GenBank accession no. AY339865.