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. 2006 Oct 18;81(1):95–105. doi: 10.1128/JVI.01608-06

FIG. 6.

FIG. 6.

E1B-156R does not relocalize p53 in transfected H1299 cells. H1299 cells were transfected with plasmids expressing p53 (1 μg pC53-SN3), E1B-55K (1 μg pE1B-55K ΔSD #1415), or E1B-156R (1 μg pE1B-156R #1326) or a combination of p53 and one of the E1B proteins. The exogenous proteins expressed in each sample are noted above the corresponding subset of triplet pictures. Images a to c were derived from untransfected (mock) cells and show the level of unspecific background staining. The cells were double labeled in situ with the anti-E1B mouse antibody 2A6 and the p53-specific antibody FL-393. These were detected with Texas Red- and FITC-conjugated secondary antibodies, respectively. Anti-p53 (green; a, d, g, j, m, and p) and anti-E1B (red; b, e, h, k, n, and q) staining patterns are visible. An overlay of both patterns (merge) is displayed in panels c, f, i, l, o, and r. Boundaries of nuclei are marked with dashed lines. Magnification was ×7,600.