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. 2006 Oct 18;81(1):95–105. doi: 10.1128/JVI.01608-06

FIG. 8.

FIG. 8.

E1B-156R can bind human Daxx. (A) H1299 cells were transfected with plasmids expressing human Daxx (25 μg phDaxx-HA #1607), E1B-55K ΔSD (15 μg pE1B-55K ΔSD #1415), and E1B-156R (15 μg pE1B-156R #1326), and total cell extracts were prepared. A plus sign indicates the use of a given plasmid in the corresponding lane. Daxx and the E1B proteins were detected by immunoblotting. A Western blot assay of β-actin was included as a loading control. (B) The same lysates were used to immunoprecipitate (IP) Daxx with the anti-Daxx antibody. The protein could be detected by Western blot assays in all samples using the same antibody. E1B-156R and E1B-55K were coimmunoprecipitated with Daxx and detected by Western blot assays using the 2A6 antibody.