Skip to main content
. 2006 Oct 18;81(1):30–41. doi: 10.1128/JVI.01434-06

FIG. 5.

FIG. 5.

Contribution of NLS2 to vRNA transcription. (A) Schematic diagram of NLS2-NP-NLS2mut and NLS2mut-NP-NLS2mut. The NLS2 or NLS2mut motif was added to the amino terminus of NP-NLS2mut. The alanine replacements introduced to abolish the nuclear transport activity of NLS2 are in red. (B) Subcellular localization of mutant NPs. Plasmids for the expression of wild-type and mutant NP were transfected into COS-7 cells. At 12 h posttransfection, cells were fixed and incubated with an anti-NP antibody. The lower parts are differential interference contrast images. Arrowheads point to nucleoli. Scale bars, 10 μm. (C) NP support of vRNA transcription as measured by reporter luciferase activity. Plasmids for the expression of wild-type NP and the indicated mutant NPs were transfected into 293 cells together with protein expression plasmids for viral polymerase subunits and pPolI/NP(0)Fluc(0). At 12 h (white bar) and 24 h (black bar) posttransfection, cells were subjected to a dual-luciferase assay. Relative firefly luciferase activity, normalized to Renilla luciferase activity, is shown. Error bars indicate the standard error of three independent experiments.