FIG. 7.

Total ion chromatogram showing the metabolites produced from 2,2′-CB by E. coli expressing either S100 BphAE or the indicated S100 variants. IPTG-induced cells were incubated for 18 h with 100 nmol 2,2′-CB and then extracted with ethyl acetate, and the metabolites were derivatized with butylboronate for GC-MS analysis. Controls of E. coli cells incubated in identical conditions produced similar amounts of 2,3-dihdyro-2,3-dihydroxybiphenyl when biphenyl was the substrate. Furthermore, SDS-PAGE of the cell extracts showed similar levels of production of BphAE for all strains.