FIG. 6.

Rgp activity in cellular compartments. Cellular fractions of various mutants were assayed for Arg-specific activity using 20 μl of each sample and synthetic substrates as per Materials and Methods. Total Rgp activity in the RgpB⁺ control mutant was set at 100 activity units. Data were obtained from duplicate enzymatic measurements from two independent runs. Differences in the total arginine-specific activity of each strain against the RgpB⁺ mutant were statistically analyzed using one-way ANOVA with Bonferroni's correction. Values that were significantly different from the value for the RgpB⁺ mutant are indicated by asterisks (**, P < 0.01; ***, P < 0.001).