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Cellular localization of RgpB. Cellular fractionation techniques were employed to separate cellular compartments into whole cells (A) and periplasm (B), cytoplasm (C), and membrane (D) fractions. Fifty-microliter samples from each fraction were separated by reducing SDS-PAGE and transferred onto nitrocellulose membranes prior to detection with anti-RgpB MAb. The positions of molecular mass markers (in kilodaltons) are indicated to the left of the gel.
