TABLE 2.

RPP cleaves mitochondrial presequence peptides

Peptidea	Peptide sequence and sites of cleavage by RPP and MPPb	Degradation by RPPc (%)
1	LSALARPVGAA↓LRRS↓↑FSTSAQNNAKVA	59
2	MLAAKNILNRSSLSSS↓↑FRIATRL↓QSTKVQGSA	39
3	LSRVAKRA↓↑FSSTVANP	20
4	MLRAA↓LTTVRRGPRLSR↓L↓↑LSAAATSAVPA	17
5	MFS↓KLAHL↓Q↓RPA↓VLSRG↑VHSSVASA	17
6	MLSAARLQFAQGSVRR↓↑LTVSARDAPTKISTLA	9.5
7	GRWRLLVRPRAGAGG↓LRGSRGPGLGGGAVATRT↑LSV	7.3
8	LSLRQSIRFFKPATRT↑LCSSTYLL	<1

^aPeptide 1, mouse MDH_2-28; 2, yeast heat shock protein SSC1 (amino acids 1 to 32); 3, yeast MDH_2-17; 4, mouse aldehyde dehydrogenase (amino acids 1 to 29); 5, human ornithine aminotransferase (amino acids 1 to 25); 6, yeast ubiquinol cytochrome c reductase subunit 2 (amino acids 1 to 32); 7, bovine ADX_18-57; 8, yeast COXIV_2-25.

^bArrows (↓) denote RPP cleavage sites for peptides that can be detected or MPP cleavage sites (↑).

^cCleavage efficiencies are determined as described in Materials and Methods.