TABLE 2.
Similarity to protein sequences of ORFs in cosmid clone 49 derived from M. intracellulare serotype 7 strain ATCC 35847
| ORF | Predicted molecular mass (kDa) | Predicted pI | Similar protein (GenBank accession no.) | E value | Amino acid identity (% matched/total no. of residues)a |
|---|---|---|---|---|---|
| MtfB | 30.5 | 5.09 | Methyltransferase MtfB (Q9WVW3) | E-143 | 90.4/272 |
| GtfA | 46.5 | 5.84 | Glycosyltransferase GtfA (O68999) | 0.0 | 90.7/421 |
| RtfA | 46.9 | 7.76 | Rhamnosyltransferase A (Q6U848) | 0.0 | 98.6/419 |
| MtfC | 30.0 | 5.61 | Methyltransferase MtfC (Q8GEA1) | E-142 | 92.1/265 |
| MtfD | 32.9 | 4.89 | Methyltransferase MtfD (Q9WW56) | E-147 | 91.5/272 |
| DhgA | 27.3 | 5.96 | Dehydrogenase DhgA (AY130970) | E-120 | 85.8/260 |
| GtfB | 45.5 | 6.35 | Glycosyltransferase GtfB (Q9WW66) | 0.0 | 85.4/419 |
| ORF 1 | 45.1 | 6.10 | Glycosyltransferase GtfA (O68999) | E-137 | 58.6/420 |
| ORF 2 | 30.9 | 8.14 | Methyltransferase (Q885B6) | 5E-47 | 44.3/237 |
| ORF 3 | 36.6 | 6.01 | Putative glycosyltransferase Rv1516c (P71795) | E-118 | 66.4/307 |
| ORF 4 | 40.4 | 4.92 | Aminotransferase/DegT_DnrJ_EryC1 (Q50723) | E-154 | 75.5/351 |
| ORF 5 | 36.1 | 6.43 | Putative enzyme, similar to large subunit of carbamoyl phosphate synthase protein (Q92VH3) | 1E-39 | 36.1/321 |
| ORF 6 | 33.8 | 5.48 | Putative nucleotide sugar epimerase (Q92VH2) | 2E-32 | 30.0/297 |
| ORF 7 | 27.6 | 5.91 | Metallophosphoesterase (Q9HMV3) | 5E-16 | 27.5/218 |
| ORF 8 | 78.5 | 8.91 | Putative acyltransferase (Q73SR3) | 0.0 | 49.1/721 |
| ORF 9 | 37.6 | 8.77 | Putative glycosyltransferase Rv1518 (Q50590) | E-101 | 61.8/304 |
| DrrC | 28.5 | 11.69 | Daunorubicin resistance protein C (Q9XCF9) | E-123 | 83.7/263 |
a
Identity values were calculated by using BLASTX searches.