TABLE 1.
Experimental conditionsa | N14CO− uptake (% of control) | 14C fixation (% of control) |
---|---|---|
Light (control) | 100 | 100 |
Dark | 6.3 | 2.4 |
Light + 25 μM DCMU | 7.9 | 2.2 |
Light + 15 mM glycolaldhyde | 59.1 | 5.6 |
Light + 15 mM oxalate | 88 | 99.7 |
Light + 1 mM ethoxyzolamide | 39 | 32.3 |
NCO− uptake and C fixation were measured by the silicone fluid filtering centrifugation technique using method A. Cells grown in standing culture were incubated with 1 mM KO14CN for 2 min in BTP/HCl buffer, pH 8.0, containing 25 mM NaCl at 30°C and illuminated at 300 μmol m−2 s−1. Data are the average of 5 experiments in triplicate ±6.5%.