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. 2006 Nov 22;189(3):1013–1024. doi: 10.1128/JB.01328-06

TABLE 1.

Characterization of NCO uptake and metabolism in Synechococcus sp. strain UTEX 625

Experimental conditionsa N14CO uptake (% of control) 14C fixation (% of control)
Light (control) 100 100
Dark 6.3 2.4
Light + 25 μM DCMU 7.9 2.2
Light + 15 mM glycolaldhyde 59.1 5.6
Light + 15 mM oxalate 88 99.7
Light + 1 mM ethoxyzolamide 39 32.3
a

NCO uptake and C fixation were measured by the silicone fluid filtering centrifugation technique using method A. Cells grown in standing culture were incubated with 1 mM KO14CN for 2 min in BTP/HCl buffer, pH 8.0, containing 25 mM NaCl at 30°C and illuminated at 300 μmol m−2 s−1. Data are the average of 5 experiments in triplicate ±6.5%.