TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA gyrA96 thi-1 hsdR17 supE44 relA1 deoR | Invitrogen |
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| B. cenocepacia strains | ||
| K56-2 | Cystic fibrosis respiratory isolate | 28 |
| K56-R2 | cepR::Tn5-OT182 derivative of K56-2, Tcr | 23 |
| K56-dI2 | ΔcepI derivative of K56-2 | 31 |
| Plasmids | ||
| pCR2.1TOPO | Cloning vector for PCR products, Apr Kmr | Invitrogen |
| pBS7 | pCR2.1TOPO containing 1.3-kb ndh fragment | 14 |
| pMS402 | lux-based promoter reporter plasmid, Kmr Tpr | 12 |
| pCC1 | pMS402 containing a zmpA::luxCDABE fusion, Kmr Tpr | 40 |
| pSLS225 | pUCP26 with 1.5-kb SphI-KpnI fragment containing cepI, Tcr | 25 |
| pRM445 | pMS402 containing a cepR::luxCDABE transcriptional fusion, Kmr Tpr | 31 |
| pCP300 | pMS402 containing a cepI::luxCDABE transcriptional fusion, Kmr Tpr | 31 |
| pMVbcscV | pMS402 containing a bcscV::luxCDABE transcriptional fusion, Kmr Tpr | This study |
| pBS18 | pMS402 containing a katB::luxCDBAE transcriptional fusion, Kmr Tpr | |
| pVDA01 | pMS402 containing a pvdA::luxCDBAE transcriptional fusion, Kmr Tpr | This study |
| pVDO301 | pMS402 containing an orbI::luxCDBAE transcriptional fusion, Kmr Tpr | This study |
a
Ap, ampicillin; Km, kanamycin; Tp, trimethoprim; Tc, tetracycline.