TABLE 3.
C. trachomatis serovar E/Bour mutant strains used to detect LGT
| Strain | Used in cross no.: | Response toa:
|
Gene and mutation | |||
|---|---|---|---|---|---|---|
| OFX | LIN | TMP | RIF | |||
| wt | s | s | s | s | wt | |
| OFXr-1 | 1, 3, 4, 5, 6, 7 | r | s | s | s | gyrA (CT189); T249→G |
| OFXr-2 | 2 | r | s | s | s | gyrA (CT189); A247→C |
| LINr-1 | 1, 2, 5, 6, 7 | s | r | s | s | 23S rRNA (IGR610, IGR617); A2039→C |
| RIFr-1 | 3, 7 | s | s | s | r | rpoB (CT315); T1383→G |
| TMPr-1 | 4 | s | s | r | s | Not known |
a
s, sensitive, i.e., does not form normal inclusions in the presence of the specified antibiotic; r, resistant, i.e., forms normal inclusions in the presence of the specified antibiotic. Antibiotics were used at the following concentrations: OFX at 1 μg per ml, LIN at 2 μg per ml, RIF at 0.015 μg per ml, and TMP at 15 μg per ml.