. 2006 Nov 22;189(3):991–1003. doi: 10.1128/JB.00845-06

TABLE 5.

Relevant nucleotides in doubly resistant C. trachomatis strains isolated from the indicated flasks

Construct	n^a	wt sequence and relevant nt^b change
Construct	n^a	23S rRNA-1 and -2 (nt 2034-2044)^c	gyrA (nt 243-253)	rpoB (nt 1378-1388)
wt		`ACGAAAAGACC`	`AGAAAGTGTCA`	`ATGGATCAGAC`
Cross no. 1
OFX^r-1		`...........`	`......G....`	`NS`
LIN^r-1		`.....C.....`	`...........`	`NS`
OFX^r LIN^r flasks
Set B	2/10	`.....C.....`	`......G....`	`NS`
Set C	10/10	`.....C.....`	`......G....`	`NS`
Set D	10/10	`.....C.....`	`......G....`	`NS`
Cross no. 2
OFX^r-2		`...........`	`....C......`	`NS`
LIN^r-1		`.....C.....`	`...........`	`NS`
OFX^r LIN^r flasks
Set B	1/10^d	`.....C.....`	`...........`	`NS`
	1/10	`.....C.....`	`......A....`	`NS`
Set C	10/10	`.....C.....`	`....C......`	`NS`
Cross no. 3
OFX^r-1		`NS^f`	`......G....`	`...........`
RIF^r-1		`NS`	`...........`	`.....G.....`
OFX^r RIF^r flasks
Set A	5/10^e	`NS`	`......G....`	`...........`
Set B	1/10^d	`NS`	`...........`	`.....G.....`
	2/10	`NS`	`.....T.....`	`.....G.....`
Set C	10/10	`NS`	`......G....`	`.....G.....`
Cross no. 5
OFX^r-1		`...........`	`....C......`	`NS`
LIN^r-1		`.....C.....`	`...........`	`NS`
OFX^r LIN^r flasks
Set V	10/10	`.....C.....`	`....C......`	`NS`

Number of flasks yielding isolates with the mutation/total number of flasks.

nt, nucleotide.

The two 23S rRNA genes were individually sequenced (Tables 1 to 3); both of the genes in an isolate always had identical sequences.

Spontaneous OFX^r mutant that lacked a mutation in gyrA nt 1 to 438.

Spontaneous RIF^r mutants in cross no. 3 that had a G1399→A, T1432→G, T1432→C, G1441→T, or C1525→T mutation in rpoB.

NS, not sequenced.