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. 2006 Nov 22;189(3):918–928. doi: 10.1128/JB.01292-06

FIG. 1.

FIG. 1.

Characterization of the WS/DGAT homologues AtfA1 and AtfA2 from A. borkumensis SK2. (A) Organization of the atfA1 and atfA2 gene loci. ABO_2740, hypothetical protein gene; aceA, isocitrate lyase gene; ABO_2743, hypothetical protein gene; cspG, cold-shock domain family protein gene; ABO_2745, hypothetical protein gene; ABO_1806, AMP-binding family protein gene; ABO_1805, hypothetical protein gene; ABO_1803, hypothetical protein gene; ABO_1802, CinA domain protein gene; recA, RecA protein gene. (B) Multiple amino acid sequence alignment of the WS/DGAT enzyme AtfA from A. baylyi strain ADP1 and AtfA1 and AtfA2 from A. borkumensis SK2. Analysis was done using the CLUSTAL W program (37). Residues identical in all sequences are shaded in light gray, and those conserved only in AtfA and AtfA1 are shaded in dark gray. A putative active site motif is boxed. (C) Transcription analysis of atfA1 and atfA2. Expression of atfA1 and atfA2 was analyzed by RT-PCR in samples derived from cells cultivated with 1% (wt/vol) sodium pyruvate as the carbon source. St, PstI-digested λ DNA. +, RT-PCR assay; −, control for DNA contamination experiments.