TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant propertiesa | Source or reference |
|---|---|---|
| Alcanivorax borkumensis strains | ||
| SK2 | Type strain, wild type | 42; DSM 11573 |
| atfA1ΩKm | atfA1 disruption mutant; Kmr; derivative of SK2 | This study |
| atfA2ΩSm | atfA2 disruption mutant; Smr; derivative of SK2 | This study |
| atfA1ΩKmatfA2ΩSm | atfA1 and atfA2 double disruption mutant; Kmr; Smr; derivative of atfA1ΩKm | This study |
| Alcanivorax jadensis T9 | Type strain, wild type | 5, 11; DSM 12178 |
| Marinobacter hydrocarbonoclasticus | Type strain, wild type | 15; DSM 8798 |
| Thalassolituus oleivorans | Type strain, wild type | 40; DSM 14913 |
| Escherichia coli strains | ||
| BL21(DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3) | Novagen, Madison, WI |
| S17-1 | recA1 thi1 hsdR17 (rK− mK+) proA tra-RP4 | 32 |
| Plasmids | ||
| pET23a(+) | Apr; T7 promoter-based expression vector | Novagen, Madison, WI |
| pET23a::atfA1 | Derivative of pET23a containing the atfA1 gene as a 1.4-kbp HindIII/XhoI fragment; Apr | This study |
| pET23a::atfA2 | Derivative of pET23a containing the atfA2 gene as a 1.4-kbp HindIII/XhoI fragment; Apr | This study |
| pET23a::atfA1ΩKm | ΩKmr cassette cloned into StuI site of pET23a::atfA1; Apr; Kmr | This study |
| pET23a::atfA2ΩSm | ΩSmr cassette cloned into EheI site of pET23a::atfA2; Apr; Smr | This study |
| pSKsymΩKm | Apr; Kmr; contains ΩKmr cassette | 27 |
| pCDFDuet-1 | Smr; source for ΩSmr cassette construction | Novagen, Madison, WI |
| pSUP202 | Apr; Cmr; Tcr; ColE1 origin; mob site; unable to replicate in A. borkumensis | 32 |
| pSUP202::pET23a::atfA1ΩKm | Fusion of pSUP202 and pET23a::atfA1ΩKm via HindIII sites; mob site; Apr; Cmr; Kmr | This study |
| pSUP202::pET23a::atfA2ΩSm | Fusion of pSUP202 and pET23a::atfA2ΩSm via HindIII sites; mob site; Apr; Cmr; Smr | This study |
a
Tc, tetracycline.