TABLE 3.

WS and DGAT activities in A. borkumensis and E. coli strains^a

Strain	Sp act (pmol mg−1 min−1) of enzyme:
	WS	DGAT
E. coli BL21(DE3)
pET23a	0.4 ± 0.1	0.2 ± 0.0
pET23a::atfA1	308.2 ± 37.9	131.7 ± 4.3
pET23a::atfA2	237.0 ± 30.5	4.6 ± 0.8
pET23a::atfA1ΩKm	1.0 ± 0.5	0.3 ± 0.1
pET23a::atfA2ΩSm	4.9 ± 0.3	0.3 ± 0.1
A. borkumensis
SK2	64.0 ± 8.1	17.2 ± 0.3
atfA1ΩKm	11.3 ± 1.4	2.5 ± 0.2
atfA2ΩSm	38.5 ± 1.2	10.1 ± 1.6
atfA1ΩKmatfA2ΩSm	0.4 ± 0.2	1.6 ± 0.8

^aAssays were done employing crude extracts corresponding to 50 μg total protein for recombinant E. coli strains or 100 μg total protein for pyruvate-grown A. borkumensis strains. Data are mean values ± standard deviations from experiments done in triplicate. Control experiments without addition of acyl acceptor substrates revealed WS activities of 14.5 and 6.4 pmol mg⁻¹ min⁻¹ and DGAT activities of 10.3 and 0.3 pmol mg⁻¹ min⁻¹ for E. coli strains expressing atfA1 and atfA2, respectively. One picomole per milligram per minute corresponds to 117.66 and 235.32 cpm for E. coli and A. borkumensis strains, respectively.