TABLE 2.
Genes under Zur transcriptional controla
| Locusb | Gene | q value (%)c | Fold inductiond | Gene product/function |
|---|---|---|---|---|
| Rv0105c | rpmB1 | 0 | 2.7 | Probable 50S ribosomal protein L28 RpmB1 |
| Rv0106 | 0 | 8.1 (39.6) | Similar to low-affinity zinc transporter YciC | |
| Rv0232 | 0 | 1.9 | Probable transcriptional regulator (TetR family) | |
| Rv0280 | ppe3 | 0 | 9.6 (2.3) | PPE family protein |
| Rv0281 | 0 | 3.6 | Unknown; possibly O-methyltransferase involved in polyketide biosynthesis | |
| Rv0282e | ND (5.9) | Unknown; membrane protein similar to ESX-1 secretion system member | ||
| Rv0283 | 0 | 4.3 | Unknown; membrane protein similar to ESX-1 secretion system member | |
| Rv0284 | 0 | 3.0 | Unknown; membrane protein, contains putative FtsK/SpoIIIE family protein domain similar to ESX-1 secretion system member | |
| Rv0285 | pe5 | 0 | 3.4 | PE family protein |
| Rv0286 | ppe4 | 0 | 3.3 | PPE family protein |
| Rv0287 | esxG | 0 | 3.0 | ESAT-6-like protein |
| Rv0288 | esxH | 0 | 3.2 | ESAT-6-like protein |
| Rv0289 | 0 | 3.1 | Unknown; similar to ESX-1 secretion system member | |
| Rv0290 | 0 | 3.7 | Unknown; similar to ESX-1 secretion system member | |
| Rv0291 | mycP3 | 0 | 3.1 | Peptidase of subtilase family/membrane-anchored mycosin, similar to ESX-1 secretion system member |
| Rv0292 | 0 | 3.3 | Unknown; transmembrane protein similar to ESX-1 secretion system member | |
| Rv1195 | pe11 | 0 | 2.4 | PE family protein |
| Rv1857 | modA | 0 | 1.9 (3.3) | Lipoprotein involved in transport of molybdenum into the cell |
| Rv1870c | 0.6 | 2.1 | Unknown | |
| Rv2055c | rpsR2 | 0 | 12.8 | Probable ribosomal protein S18 RpsR2 |
| Rv2056c | rpsN2 | 0 | 22.5 | Probable ribosomal protein S14 RpsN2 |
| Rv2057c | rpmG1 | 0 | 23.3 | Probable ribosomal protein L33 |
| Rv2058c | rpmB2 | 0 | 30.1 (125) | Probable 50S ribosomal protein L28 RpmB2 |
| Rv2059 | 0 | 5.8 (22.5) | Similar to periplasmic metal binding proteins of ABC transport systems | |
| Rv2060 | 0 | 6.3 | Similar to ZnuB, ABC-type Mn2+/Zn2+ transport systems, permease components | |
| Rv2990c | 0 | 4.1 | Unknown | |
| Rv3017c | esxQ | 1.0 | 2.1 (2.8) | ESAT-6-like protein |
| Rv3019c | esxR | 0 | 3.9 | ESAT-6-like protein |
| Rv3020c | esxS | 0 | 2.5 | ESAT-6-like protein |
| Rv3022c | ppe48 | 0 | 2.2 | PPE family protein |
| Rv3229c | 0 | 2.9 | Involved in lipid metabolism, possible fatty acid desaturase | |
| Rv3612c | 0 | 2.1 | Unknown; up-regulated after 4 h and 24 h of starvation |
a
Genes were included in the table if their q value was ≤1 and fold induction was ≥1.9.
b
Genes are annotated as described by the Pasteur Institute on TUBERCULIST (http://genolist.pasteur.fr/TubercuList/). Genes downstream of a putative Zur box are shown in boldface type.
c
False discovery rate (probability that the gene was falsely called, calculated by SAM).
d
mRNA levels in the zur mutant strain ST129 divided by those in the wild-type strain H37Rv. Values in parentheses represent the fold induction obtained by quantitative RT-PCR. ND, not determined.
e
Included in the table based on results obtained by RT-PCR, as no data were obtained from DNA microarray experiments.