FIG. 6.

Splicing of the nrdB intron in different growth phases. (a) Cultures of JM101 containing either pBS5 or pBS5 Δ45-129;ΔCry-P1 were grown at 37°C, and samples were taken at the indicated time points after induction of transcription. (b) After an overnight incubation, part of the pBS5 culture was centrifuged and resolved in fresh medium and incubation was continued. Splicing efficiency at the different time points was measured by limited primer extension. RNA was prepared from equal densities of cells for each sample. Open symbols in the graphs represent splicing efficiency, and closed symbols represent growth. Error bars show the standard error of the mean. wt, wild type.