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. 1999 Sep 14;96(19):10857–10862. doi: 10.1073/pnas.96.19.10857

Figure 1.

Figure 1

Northern analysis of insulin mRNA in HIT-T15 cells cultured in various concentrations of NAC and AG for 35 weeks. The autoradiogram depicts representative experiments from passage 105 chronically cultured with NAC and from passage 104 chronically cultured with AG. The relative density of insulin mRNA to β-actin is normalized to that of control data from HIT-T15 cells at passage 75. NAC 0, 0.15 ± 0.2; NAC 0.1, 0.16 ± 0.01; NAC 0.5, 0.57 ± 0.19*; NAC 1, 0.53 ± 0.13*; AG 0, 0.16; AG 0.01, 0.61). Data are presented as mean ± SEM for three independent experiments at passages 105, 107, and 108 in the NAC study, and are presented as mean of two independent experiments at passages 97 and 104 in the AG study (*, P < 0.01 vs. NAC 0).