TABLE 2.
Properties of cyclohexa-1,5-diene-1-carbonyl-CoA hydratases from G. metallireducens (BamRGeo) and S. aciditrophicus (BamRSyn)
| Hydratase | Substrate used
|
Substrates not used | Km (μM) | Sp act (μmol min−1 mg−1)
|
Catalytic no. for hydration (s−1) | Subunit compositiona | ||
|---|---|---|---|---|---|---|---|---|
| Hydration | Dehydration | Hydration | Dehydration | |||||
| BamRGeo | Dienoyl-CoA | 6-OH-cyclohexenoyl-CoA | Cyclohex-1-ene-1-carbonyl-CoA, crotonyl-CoA | 85 ± 20 (dienoyl-CoA), 50 ± 20 (6-OH-cyclohexenoyl-CoA) | 550 | 730 | 280 | α2 or α3 |
| BamRSyn | Dienoyl-CoA | 6-OH-cyclohexenoyl-CoA | Cyclohex-1-ene-1-carbonyl-CoA, crotonyl-CoA | 30 ± 10 (dienoyl-CoA) | 350 | 175 | α4 | |
a
The subunit was 30 kDa.