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Purification of acetone monooxygenase from Gordonia sp. strain TY-5
| Purification step | Total activity (U) | Total protein (mg) | Sp act (U/mg of protein) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Cell extract | 9.77 | 643 | 0.015 | 100 | 1 |
| DEAE-Toyopearl | 6.47 | 140 | 0.046 | 66.2 | 3.1 |
| HiPrep 16/10 Phenyl FF | 1.22 | 8.1 | 0.15 | 12.5 | 10.0 |
| Superose 6 10/300 GL | 1.21 | 3.0 | 0.40 | 12.4 | 26.7 |
| MonoQ HR 5/5 | 0.52 | 0.85 | 0.61 | 5.3 | 40.7 |
