Alignment of amino acid sequences of GerD from the various Bacillus species. The species compared were B. subtilis, Bacillus licheniformis, Geobacillus stearothermophilis, Bacillus anthracis, Bacillus thuringiensis, and Bacillus cereus. Gray shading indicates similar residues, and black shading indicates identical residues. The asterisk denotes the cysteine residue that is the putative site for diacylglycerol addition. The A and B over the sequences delineate the region of gerD coding sequence deleted and replaced with an antibiotic resistance gene in the ΔgerD strains used in this work.