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Rates of germination of wild-type and gerD spores measured by microscopy. Wild-type (PS832) and ΔgerD (FB62) spores were spotted on LB medium agar slides with l-alanine and kanamycin, and spore germination was assessed by examining ∼100 spores by phase-contrast microscopy as described in Materials and Methods. ○, wild-type spores; •, ΔgerD spores.
