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Rates of germination of wild-type and ΔgerD spores with (A) dodecylamine or (B) Ca2+-DPA. Wild-type (PS832) and ΔgerD (FB62) spores were germinated with 1 mM dodecylamine (A) or with Ca2+-DPA (B), and spore germination was measured either by following DPA release (dodecylamine germination) or flow cytometry after staining with Syto 16 (Ca2+-DPA germination), as described in Materials and Methods. ○, wild-type spores; •, ΔgerD spores.
