TABLE 1.
Bacillus subtilis strains used
| Strain | Genotype | Sourcea or reference |
|---|---|---|
| PS832 | Wild type | Laboratory stock |
| FB10 | gerBB* | 25 |
| FB62 | ΔgerD::spc | 10 |
| FB87 | ΔgerB::cat ΔgerK::erm | 26 |
| PS3476 | PsspD-gerA | 6 |
| PS3502 | PsspD-gerB* | 6 |
| PS3521 | gerBB* ΔgerA::neo | PS3609→FB10 |
| PS3609 | ΔgerA::neo | 10 |
| PS3651 | ΔgerA::neoΔgerK::erm | 2 |
| PS3665 | gerBB* ΔgerA::neo ΔgerK::erm | 2 |
| PS3926 | ΔgerA::neoΔgerD::spc | FB62→PS3609 |
| PS3927 | ΔgerB::cat ΔgerK::erm ΔgerD::spc | FB62→FB87 |
| PS3928 | ΔgerA::neo ΔgerB::cat ΔgerK::erm ΔgerD::spc | PS3609→PS3927 |
| PS3929 | gerBB* ΔgerD::spc | FB62→FB10 |
| PS3931 | gerBB* ΔgerA::neo ΔgerD::spc | FB62→PS3521 |
| PS3932 | gerBB* ΔgerA::neo ΔgerK::erm ΔgerD::spc | PS3657→PS3931 |
| PS3938 | ΔgerA::neo ΔgerK::erm ΔgerD::spc | PS3657→PS3931 |
| PS3940 | PsspD-gerA ΔgerD::spc | FB62→PS3476 |
| PS3945 | ΔgerA::neo ΔgerB::cat ΔgerK::erm | PS3609→FB87 |
| PS3946 | PsspD-gerB* | pVK73→PS3502 |
| PS3947 | PsspD-gerB* ΔgerD::spc | FB62→PS3946 |
| PS3980 | ΔgerD::cam | pΔgerD::cam→PS832 |
| PS3981 | gerD-spc | pgerDSpr→PS3980 |
| PS3982 | gerDC20A-spc | pgerDA20Spr→PS3980 |
| PS3983 | gerDΔSP-spc | pgerDΔSPSpr→PS3980 |
| PS3987 | ΔgerD amyE::gerD | pΔamyE::gerD→PS3980 |
| PS3988 | ΔgerD amyE::PsspB-gerD | pΔamyE::PsspB-gerD→PS3980 |
| PS3989 | amyE::gerD | pΔamyE::gerD→PS3981 |
| PS3990 | amyE::PsspB-gerD | pΔamyE::PsspB-gerD→PS3981 |
| PS3991 | amyE::gerDC20A | pΔamyE::gerDAla20→PS3981 |
| PS3992 | amyE::gerDΔSP | pΔamyE::gerDΔSP→PS3981 |
| PS3993 | gerDC20AamyE::gerD | pΔamyE::gerD→PS3982 |
| PS3994 | gerDC20AamyE::PsspB-gerD | pΔamyE::PsspB-gerD→PS3982 |
| PS3995 | gerDΔSPamyE::gerD | pΔamyE::gerD→PS3983 |
| PS3996 | gerDΔSPamyE::PsspB-gerD | pΔamyE::PsspB-gerD→PS3983 |
a
Strains made in this work were constructed by transformation of the strain to the right of the arrow with chromosomal or plasmid DNA from the strain to the left of the arrow. The abbreviation ΔSP denotes a gerD gene lacking the region coding for amino acids 7 to 17, which is a likely SP.