TABLE 2.
Germination of gerD spores with various genetic backgroundsa
| Genetic background (strainsb) | Germinant | Rate of germination (%/h)
|
|
|---|---|---|---|
| With gerD | Without gerD | ||
| Wild type (PS832/FB62) | Ala | 110 | <5 |
| Wild type (PS832/FB62) | AGFK | 60 | <5 |
| Wild type (PS832/FB62) | LB medium | 87 | <5 |
| ΔgerA (PS3609/PS3926) | Ala | <5 | <5 |
| ΔgerA (PS3609/PS3926) | AGFK | 61 | 6 |
| ΔgerA (PS3609/PS3926) | LB medium | <12 | <5 |
| ΔgerK ΔgerB (FB87/PS3927) | Ala | 105 | 6 |
| ΔgerK ΔgerB (FB87/PS3927) | AGFK | 6 | <5 |
| ΔgerK ΔgerB (FB87/PS3927) | LB medium | 73 | 12 |
| gerBB* (FB10/PS3929) | Ala | 239 | 15 |
| gerBB* (FB10/PS3929) | AGFK | 219 | 10 |
| gerBB* ΔgerA (PS3521/PS3931) | Ala | 83 | 5 |
| gerBB* ΔgerA (PS3521/PS3931) | AGFK | 271 | 14 |
| gerBB* ΔgerA ΔgerK (PS3665/PS3932) | Ala | 104 | 7 |
| gerBB* ΔgerA ΔgerK (PS3665/PS3932) | AGFK | 78 | 7 |
| PsspD-gerA (PS3476/PS3940) | Ala | 200 | 23 |
| PsspD-gerA (PS3476/PS3940) | AGFK | 28 | 5 |
| PsspD-gerB* (PS3946/PS3947) | Ala | 247 | 6 |
| PsspD-gerB* (PS3946/PS3947) | AGFK | 257 | <5 |
a
Spore germination was induced by addition of l-alanine (Ala), AGFK, or LB medium; germination was measured by monitoring the OD600 of cultures, and rates of spore germination were calculated as described in Materials and Methods.
b
The first strain listed contains gerD, and the second has the same genetic background but without gerD.