TABLE 4.

Germination of ΔgerD and wild-type spores prepared and germinated in various media^a

Germinant	Rate of spore germination (%/h) for indicated strainb in:
	2× SG		RM		SMM + CAA
	PS832	FB62	PS832	FB62	PS832	FB62
LB medium	87	<5	NDc	ND	ND	ND
Ala	ND	ND	34	<5	ND	ND
AGFK	ND	ND	56	<5	ND	ND
SMM plus Ala	ND	ND	ND	ND	64	<5
SMM plus AGFK	ND	ND	ND	ND	90	<5

^aSpores were prepared in 2× SG medium, RM, or SMM plus 0.1% Casamino Acids (CAA), and spore germination was carried out with either LB medium, L-alanine (Ala), AGFK, or SMM with 10 mM L-alanine or with AGFK. Spore germination was measured by monitoring the OD₆₀₀ of cultures as described in Materials and Methods. Rates of germination are given as the percentages of spores that germinate in 1 hour as described in Materials and Methods.

^bPS832 is the wild type, and FB62 lacks gerD.

^cND, not done.