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Growth curves generated for M. avium subsp. paratuberculosis strains 187 and K-10 in Middlebrook 7H9 broth supplemented with Tween 80 and ferric mycobactin J. On the basis of their rates of growth, strain K-10 was harvested on day 18 (A540, 0.235) and strain 187 was harvested on day 22 (A540, 0.232).
