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. 2006 Nov 17;189(3):872–879. doi: 10.1128/JB.01398-06

FIG. 3.

FIG. 3.

Identification of a new trpG promoter. (A) Primer extension mapping of the 5′ end of the trpG transcript. Total RNA was isolated from a WT B. subtilis strain, hybridized to an end-labeled DNA primer, and subsequently extended with MMLV reverse transcriptase. Sequencing reactions were performed using the same end-labeled DNA primer. An arrow marks the single reverse transcriptase product corresponding to the 5′ end of the transcript originating from PtrpG. (B) σA trpG promoter sequence. The trpG promoter sequences (−35 and −10) and the transcription start site (+1) are marked. (C) In vitro transcription analysis of the trpG promoter, using σA-containing B. subtilis RNAP. Lane 1, no-template control; lane 2, DNA restriction fragment template giving rise to a 150-nt trpG transcript; lane 3, PCR-derived template giving rise to a 113-nt trpG transcript; lane 4, PCR-derived template giving rise to a 139-nt control transcript.